Taxon:
Tuber melanosporum
Total Characters: 64
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Images:
Tuber melanosporum.jpg
Tuber melanosporum ascocarp.jpg
Tuber melanosporum cistidi.jpg
Tuber melanosporum micorriza.jpg
Tuber melanosporum spores.jpg
#1.
(Nomenclature, comments, associations, bibliography)
Tuber melanosporum Vittad. Monographia Tuberacearum :36,plII fig.3 (1831)
#2.
Ascocarp (shape)
[
Image1
]
1.
subglobose
3.
lobed
#3.
Ascocarp orifice or cavity (presence)
[
Image1
]
1.
absent
#4.
Ascocarp (dimension) cm in diameter
Range:
1-7
Upper limit:
10
#5.
Ascocarp (surface color)
5.
brown
13.
black
#6.
Ascocarp (surface configuration)
[
Image1
]
2.
with warts
#7.
Ascocarp warts (with lens, type)
[
Image1
]
2.
angulated
#8.
Ascocarp peridium (surface fine texture)
#9.
Ascocarp color of spore-bearing tissue (at maturity)
6.
brown
9.
purple
10.
black
#10.
Ascocarp veins (gleba)
[
Image1
]
2.
narrow and numerous
#11.
Peridium (thickness) micron
Range:
400-700
#12.
Peridium outermost layers (thickness) micron
Range:
200-300
#13.
Peridium (outermost layers color)
3.
red-brown
<only the first layers of cells>
#14.
Peridium anatomical structure (outermost layers)
[
Image1
]
2.
pseudoparenchimatic with roundish cells
<in the middle layer the cells are radial enlonged>
#15.
Peridium cell tangential dimension (outermost layers) micron
Range:
5-15
#16.
Peridium cell radial dimension (outermost layers) micron
Range:
4-10
#17.
Peridium innermost layers (thickness) micron
Range:
200-400
#18.
Peridium color (innermost layers)
1.
hyaline
<yellowish>
2.
yellow-brown
#19.
Peridium anatomical structure (innermost layers)
[
Image1
]
2.
pseudoparenchimatic with roundish cells
3.
plectenchymatic
#20.
Peridium cell tangential dimension (innermost layers) micron
Range:
4-8
#21.
Peridium cell radial dimension (innermost layers) micron
Range:
4-8
#22.
Peridium external cystidia (presence)
2.
absent
#23.
Cystidia length micron
#24.
Cystidia basal diameter micron
#25.
Cystidia septa (presence)
#26.
Cystidia (septa number)
#27.
Sterile veins (thickness) micron
#28.
Sterile veins (anatomical features)
2.
plectenchimatic
#29.
Fertile veins (thickness) micron
#30.
Asci (shape)
[
Image1
]
2.
globose
#31.
Asci major axis (dimension) micron
Range:
90-140
#32.
Asci minor axis (dimension) micron
Range:
80-120
#33.
Asci number of spores per ascus (at maturity)
Range:
1-6
#34.
Asci (spore arrangement)
1.
irregularly clustered
#35.
Asci iodine reaction (in Melzer's reagent)
#36.
Asci color in KOH
#37.
Spores (shape)
[
Image1
]
3.
ellipsoid
#38.
Spores major axis (lenght excluding ornamentation in 4-spore asci) micrometer lenght
Mean:
32
Range:
29-35
#39.
Spores minor axis (lenght excluding ornamentation in 4-spore asci) micrometer lenght
Mean:
23
Range:
20-26
#40.
Spores color (at maturity)
4.
dark brown
#41.
Spores (ornamentation at maturity)
[
Image1
]
1.
spines
#42.
Spores ornamentation length micron
[
Image1
]
Range:
2-3
#43.
Spores mesh width (ornamentation) micron
[
Image1
]
#44.
Spores number of meshes along the major axis (ornamentation)
#45.
Habitat
1.
temperate
#46.
Putative plant host
[
Image1
]
1.
Quercus
2.
Corylus
3.
Ostrya
4.
Carpinus
5.
Tilia
11.
Cistus
12.
Pinus
#47.
ITS restriction pattern with EcoRI
3.
250-300 bp
4.
300-350 bp
#48.
ITS restriction pattern with SphI
5.
250-300 bp
6.
300-350 bp
#49.
ITS restriction pattern with HincII
2.
50-100 bp
3.
100-150 bp
6.
400-450 bp
#50.
DNA sequence ID of the ITS1-5.8S-ITS2 regions
TMU89359
#51.
Mycorrhizae emanting hyphae
1.
absent
2.
rare
#52.
Mycorrhizae anatomy of emanating hyphae: clamps (presence)
[
Image1
]
1.
absent
#53.
Mycorrhizae color of emanating hyphae
1.
hyaline
#54.
Mycorrhizae color of very tip
2.
whitish
6.
ochre, yellowish brown
10.
brown
#55.
Mycorrhizae color of older parts
6.
ochre, yellowish brown
10.
brown
#56.
Mycorrhizae cystidia (presence)
[
Image1
]
1.
absent
2.
present
#57.
Mycorrhizae (cystidia shape)
[
Image1
]
1.
needle like
#58.
Mycorrhizae color (cystidia)
2.
pale yellow
3.
ochre
#59.
Mycorrhizae intercalate swelling (cystidia, presence)
1.
absent
#60.
Mycorrhizae ramification (cystidia, presence)
[
Image1
]
1.
absent
2.
present
#61.
Mycorrhizae (cystidia ramification frequence)
2.
abundant
#62.
Mycorrhizae (cystidia ramification type)
1.
monopodial
2.
dichotomous
<apically>
#63.
Mycorrhizae (cystidia ramification position)
1.
proximal
2.
medial
#64.
Mycorrhizae anatomy of the mantle (in the plan view)
[
Image1
]
2.
pseudoparenchymatus with rounds cells
3.
pseudoparenchymatus with epidermoid hyphal cells" like a puzzle"
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