Tuber magnatum

Tuber magnatum Pico In Melethemata inauguralia de fungorum generatione et propagatione: 80(1788) specimens examined: CMI-UNIBO 1160-30 CMI-UNIBO 1208-34 CMI-UNIBO 1208-35 CMI-UNIBO 1219-36 CMI-UNIBO 1289-44

Ascocarp Morphology. Ascocarp subglobose or globose or lobed, orifice or cavity absent, 1-6(-20) cm in diameter, yellow or ochre or brown or red, smooth, peridium glabrous, color of spore-bearing tissue grey or yellow or brown (hazel) or olive, veins narrow and numerous. Peridium (253-)337-421-505(-532) micron, outermost layers (27-)45-78-111(-146) micron, hyaline or yellow-brown (yellowish), anatomical structure pseudoparenchimatic with roundish cells, cell tangential dimension (3-)9-12-15(-16) micron, cell radial dimension (3-)6-9-12(-13) micron, innermost layers (186-)251-336-421(-479) micron, color hyaline, anatomical structure plectenchymatic, cell tangential dimension 3-6-9(-10) micron, cell radial dimension 3-5-7 micron, external cystidia absent. Sterile veins 50-160 micron, plectenchimatic.

Asci ans spores Morphology. Asci globose, major axis (53-)61-69-77(-90) micron, minor axis (26-)42-53-64(-68) micron, number of spores per ascus 1-4, irregularly clustered.

Spores subglobose or globose, major axis (20-)22-25-28(-37) micrometer lenght, minor axis (16-)19-21-23(-25) micrometer lenght, color yellow or pale brown, reticulation; ornamentation length (2-)3-4-5(-7) micron; mesh width (6-)8-10-12(-16) micron; number of meshes along the major axis (2-)3(-4).

Distribution. Habitat temperate; Putative plant host Quercus, or Corylus, or Ostrya, or Carpinus, or Tilia, or Populus, or Salix.

DNA polymorphism. ITS restriction pattern with EcoRI 600-650 bp; SphI 250-300 bp, or 300-350 bp; HincII less than 50 bp, or 550-600 bp; DNA sequence ID of the ITS1-5.8S-ITS2 regions TMAJ2509.

Mycorrhizae morphology. Mycorrhizae emanting hyphae absent, or rare; anatomy of emanating hyphae: clamps absent; color of emanating hyphae hyaline; color of very tip white, or whitish, or ochre, yellowish brown; color of older parts ochre, yellowish brown, or brown; cystidia absent, or present; needle like; color hyaline; intercalate swelling absent; ramification absent; anatomy of the mantle pseudoparenchymatus with epidermoid hyphal cells" like a puzzle".


Images:
Tuber magnatum.jpg
Tuber magnatum ascocarp.jpg
Tuber magnatum ascocarp .jpg
Tuber magnatum spores.jpg

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