Tuber dryophilum

Tuber dryophilum Tul. & C. Tul. Giorn. bot. ital. ann.1, 2(1):62 (1844) specimens examined:CMI-UNIBO 1192-21 CMI-UNIBO 1196-22 CMI-UNIBO 1199-23 CMI-UNIBO 1202-24 CMI-UNIBO 1248-26 references: Pegler D.N., Spooner B., Young .W.K. (1993). British truffles. Royal Botanic Gardens, Kew.

Ascocarp Morphology. Ascocarp subglobose or globose, orifice or cavity absent, 0.5-4 cm in diameter, yellow or ochre or brown, smooth, peridium glabrous (translucid), color of spore-bearing tissue grey or brown (hazel), veins narrow and numerous. Peridium (186-)216-301-386(-452) micron, outermost layers (65-)68-112-156(-199) micron, yellow-brown, anatomical structure pseudoparenchymatic with angular cells or pseudoparenchimatic with roundish cells, cell tangential dimension (6-)9-15-21 micron, cell radial dimension (6-)7-11-15(-16) micron, innermost layers (47-)92-162-232(-292) micron, color hyaline, cell tangential dimension 3-6-9(-10) micron, cell radial dimension 3-5-7 micron, external cystidia present (rare), Cystidia length 22-31-40(-45) micron, Cystidia basal diameter 4-7-10 micron, Cystidia septa present, Cystidia 1-2. Sterile veins plectenchimatic.

Asci ans spores Morphology. Asci globose, major axis (63-)77-90-103(-126) micron, minor axis (42-)55-66-77(-90) micron, number of spores per ascus 1-4, irregularly clustered.

Spores subglobose, major axis (24-)28-31-34(-39) micrometer lenght, minor axis (24-)27-29-31(-32) micrometer lenght, color yellow or pale brown, reticulation; ornamentation length (2-)3-4-5(-7) micron; mesh width (3-)5-7-9(-15) micron; number of meshes along the major axis (3-)4-5-6(-7).

Distribution. Habitat temperate; Putative plant host Quercus, or Fagus, or Pinus.

DNA polymorphism. ITS restriction pattern with EcoRI 200-250 bp, or 300-350 bp; SphI 200-250 bp, or 250-300 bp; HincII less than 50 bp, or 500-550 bp; DNA sequence ID of the ITS1-5.8S-ITS2 regions AF003917.

Mycorrhizae morphology. Mycorrhizae emanting hyphae absent, or rare; anatomy of emanating hyphae: clamps absent; color of emanating hyphae hyaline; color of very tip whitish, or ochre, yellowish brown; color of older parts ochre, yellowish brown, or brown; cystidia absent, or present; needle like; color hyaline; intercalate swelling absent; ramification absent; anatomy of the mantle pseudoparenchymatus with epidermoid hyphal cells" like a puzzle".


Images:
Tuber dryophilum.jpg
Tuber dryophilum ascocarp.jpg
Tuber dryophilum internal peridium.jpg
Tuber dryophilum external peridium.jpg
Tuber dryophilum spores.jpg

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